Ampk

The textbook "AMPK: Methods and Protocols" offers a comprehensive examination of the latest methods for studying AMP-activated protein kinase (AMPK). It covers a variety of approaches, including computational, biochemical, biophysical, as well as cellular and ex vivo/in vivo methods. The individual chapters are thematically organized and address specific aspects such as measuring changes in cellular energy metabolism, analyzing metabolic pathways regulated by AMPK, and bioinformatics tools for identifying AMPK targets. Additionally, techniques such as CRISPR-Cas9 for AMPK knockdown, the production and crystallization of human AMPK, and cell-free assays for measuring the effects of regulatory ligands are discussed. The book is a valuable resource for students, postdoctoral researchers, and established scientists interested in the diverse field of AMPK.