Protein Lipidation Protocols

The book "Protein Lipidation Protocols" by Michael Gelb offers a comprehensive collection of techniques for studying protein lipidation, a process in which lipids are covalently attached to proteins. This collection includes innovative methods presented in a practical format, many of which are previously unpublished. The techniques cover various aspects, including glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. The included methods feature novel approaches for determining the chemical structure of GPI anchors, as well as procedures for radiolabeling prenyl groups in eukaryotic cells. The book is aimed at biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers, providing them with modern tools to explore the complex biochemistry of protein lipidation, which is significant for the development of new biopharmaceutical products, including anticancer drugs.