Purificación de anticuerpos (Ac) específicos a la proteína 'Erns'

The book "Purification of specific antibodies (Abs) to the 'Erns' protein" offers a detailed investigation into the immobilization of the Erns protein from the classical swine fever virus. It describes the use of Eupergit® CM as a carrier system to optimize protein binding through the reaction of epoxide groups with amino groups. The study documents a maximum protein immobilization of 77.7% at a reaction temperature of 60 °C over 72 hours. Furthermore, it addresses the immunopurification of a polyclonal antibody against Erns, with a recovery value of 27.5% noted at acidic pH. The biological activity of the final product was evaluated through an ELISA test, which demonstrated specific recognition of Erns and reduced cross-reactivity with E. coli extract. This specialized book is aimed at scientists and professionals working in the fields of immunology and virology.