Der Experimentator: Zellkultur

DEAR EXPERIMENTER, this book will provide you with the basic knowledge you need and help you find your way through the confusing jungle of cells, media, sera, devices and regulations. Vivid comparisons and examples, as well as a casual writing style, make it an informative and exciting read. Common problems (e.g., contamination in cell culture) are addressed in depth and suggested solutions are offered. The 2nd edition has been expanded to include the topic of cross-contamination and misidentified cell lines. Key topics such as sterile technique, subculture, adhesion and detachment, cryopreservation and long-term storage of cells as well as cell biological methods are presented in an understandable way. The use of new techniques such as siRNA is explained with an updated practical example and supplemented by a contribution on the in vitro differentiation of mesenchymal stem cells.

In addition, the book contains a revised collection of resources for obtaining cell lines, contact addresses for training courses and services related to cell culture, and lists of informative databases and useful addresses. Such an extensive resource invites you to browse and browse and provides new information even for the experienced cell culture professional. This edition provides an overview of the latest status of ordinances and regulations.

The book is aimed at students, interns, technical assistants, diploma and doctoral students, and all those who, in addition to a "cookbook" on cell culture, would like a companion in everyday laboratory life that offers help with problems, especially when Murphy's Law has struck again.