Coronaviruses

The textbook "Coronaviruses: Methods and Protocols, Second Edition" offers a comprehensive collection of protocols applicable to all members of the Coronavirinae subfamily and also transferable to other areas of virology. The new edition begins with a section on the detection, discovery, and evolution of coronaviruses, followed by discussions on the propagation and titration of coronaviruses, genome manipulation, the study of virus-host interactions, and imaging of coronavirus infections. The chapters are structured to provide an introduction to the respective topics, include a list of necessary materials and reagents, offer step-by-step, easily reproducible laboratory protocols, and provide troubleshooting tips as well as advice on avoiding common pitfalls. This book is a valuable guide for researchers working to identify and control viruses that have an increased potential to cross species barriers, as well as for the development of diagnostics, vaccines, and antiviral therapeutics needed to manage future outbreaks in humans and animals.