Ampk

The book "AMPK" offers a comprehensive examination of the latest methods for studying AMP-activated protein kinase (AMPK) through various approaches, including computational, biochemical, biophysical, as well as cellular and in vivo techniques. The chapters cover specific topics such as measuring changes in cellular energy metabolism, analyzing metabolic pathways regulated by AMPK, and identifying AMPK targets using bioinformatics tools. Additionally, innovative methods like the CRISPR-Cas9 technique for knocking down AMPK, the production and crystallization of the full human AMPK, and cell-free assays to measure the effects of regulatory ligands on AMPK are discussed. The book is written in the established format of the "Methods in Molecular Biology" series and provides a structured approach with introductions to the respective topics, lists of required materials and reagents, as well as step-by-step, reproducible laboratory protocols. It is aimed at students, postdoctoral researchers, and established scientists interested in the diverse field of AMPK.